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capable of detecting mechanical separation of the muscle layers, which was found to occur more frequently in w1118 than yw (0.415 0.04 vs. 0.00 5 0.02 mm separation, respectively, 1 week flies). Detection of mechanical separation between muscle layers via nanoindentation was modeled and verified in a microfabricated polydimethylsiloxane system. This first in situ mechanical analysis of a living myocardium revealed differences in cardiac mechanics due to age and suggest that aspects of thedoi:10.1016/j.bpj.2011.11.1923 fatcat:wcm46rudrjfkfeu7kysuuwib5q