Production of GM-CSF Mediated by Cysteine Protease of Der f in Canine Keratinocytes

Tsuyoshi KIMURA, Machiko SEKIDO, Naoki CHIMURA, Sanae SHIBATA, Naho KONDO, Harumi KAMISHINA, Hiroaki KAMISHINA, Sadatoshi MAEDA
2012 Journal of Veterinary Medical Science  
House dust mite (HDM) allergens are the most common allergens for induction of IgE-mediated hypersensitivity. Recently, epicutaneous sensitization with HDM allergens has been emphasized in the development of atopic dermatitis (AD) by producing various soluble factors in keratinocytes. Among the soluble factors, GM-CSF is a key molecule that activates Langerhans cells, antigen-presenting cells in the epidermis. In the present study, we investigated the effects of Dermatophagoides farinae (Der f)
more » ... des farinae (Der f) on GM-CSF production in a canine keratinocyte cell line, CPEK. CPEKs were found to produce GM-CSF upon stimulation by Der f. The GM-CSF production was suppressed by addition of a cysteine protease inhibitor. The present results suggest that cysteine protease-derived Der f may be an initiator of allergic inflammation by inducing the production of GM-CSF in keratinocytes.
doi:10.1292/jvms.11-0522 pmid:22446400 fatcat:f3r5dswfafbs7n2pmbtbhppyke