Congestive heart failure: increased cardiac and extracardiac atrial natriuretic peptide gene expression

J Poulos
2002 Cardiovascular Research  
Objectives: The present investigation was designed to determine if atria1 natriuretic peptide (ANP) gene expression increases in extracardiac as well as within the heart in congestive heart failure. Methods: Congestive heart failure (CHF) was induced by producing cardiac hypertrophy secondary to an aortocaval fistula in Sprague-Dawley rats. To characterize this model, control and CHF rats had cardiac catheterizations and transthoracic echocardiography. ANP messenger RNA was measured by RNAase
more » ... otection analysis in atria, ventricles, liver, colon, and stomach of CHF and sham rats and quantitated by 2-D scanning. The product of ANP gene expression was determined in each of these tissues with high performance-gel permeation chromatography. To help determine if increased degradation of atria1 natriuretic peptides occur in congestive heart failure, the circulating concentrations and the excretion of the atria1 natriuretic peptides into urine were measured by specific radioimmunoassays. Results: ANP steady-state mRNA increased 4.2 + 0.05 and 4.3 + 0.06-fold, respectively, in the antrum of the stomach and within the heart ventricle of CHF rats compared with age-matched sham rats. ANP gene expression was present but not increased in atria, liver, and gastrointestinal tract of the CHF rats. High-performance gel permeation chromatography revealed that the product of this ANP gene expression within the stomach and heart ventricle in CHF animals was the ANP prohormone. There was not any decrease in the metabolism of these peptides by the kidney in CHF. Conclusions: ANP steady-state mRNA increases in extracardiac (i.e., stomach antrum) tissue as well as in the ventricle of the heart in CHF. The product of the ANP gene expression, i.e., the ANP prohormone is the same in the extracardiac tissues as within the heart. Whether the increased extracardiac ANP steady-state mRNA and its resultant increased atrial natriuretic peptides helps prevent bowel wall edema in CHF needs to be elucidated.
doi:10.1016/s0008-6363(02)00495-9 fatcat:mp2wfkdrdreb5mvnxyilzbxbb4