Alpha-Tocopherol increases the proliferation of induced pluripotent stem cell derived neural progenitor cells

Mahboobeh Malakoutikhah, Leila Satarian, Sahar Kiani, Mohammad Javan
In addition to its antioxidant effect, Vitamin E or α-tocopherol is suggested to enhance remyelination in the animal model of non-inflammatory demyelination. In this study, the possible proliferative effect of vitamin E on human-induced pluripotent stem cell-derived neural progenitors (hiPS-NPs) and the underlying mechanisms were investigated in vitro. NPs were induced from iPS cells via 3 steps within 18 days and then characterized for NPs markers NESTIN, SOX1 and OTX2. MTT assay was used to
more » ... mpare cell populations. LY294002, U0126 and PP2 were used for selective inhibition of enzymes PI3K, MEK and Src-kinase, respectively. Vitamin E increased hiPS-NPs proliferation after 24 and 48 h exposure. The inhibition of both PI3K/Akt and Src-kinase signaling pathways counteracted the effect of vitamin E of NPs. Our data suggest that vitamin E may enhance NPs proliferation via activating PI 3K and Src-kinase and may enhance myelin repair following demyelinating Injuries.