A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is
In the present study, the surface antigen (HBsAg) of HBV was amplified by polymerase chain reaction (PCR) and cloned into an expression vector. For this purpose, HBV DNA was obtained from a patient with chronic HBV infection. Then, the PCR product of HBsAg gene of HBV was cloned into pcDNA3.1/V5 HisTOPO vector. The resulting recombinant plasmid, termed pFHBsAg, was transformed into jM109 competent bacterial cells. The presence of the HBsAg gene was confirmed by PCR screening assay. Moreover,doaj:df0afe0f868f4dd68fa5b08080f357d2 fatcat:pu6mrq5j4fcjtjp6sh4lbgpexi