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Cloning of HBsAg Gene of Hepatitis B Virus in to an Expression Vector
2003
Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi
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In the present study, the surface antigen (HBsAg) of HBV was amplified by polymerase chain reaction (PCR) and cloned into an expression vector. For this purpose, HBV DNA was obtained from a patient with chronic HBV infection. Then, the PCR product of HBsAg gene of HBV was cloned into pcDNA3.1/V5 HisTOPO vector. The resulting recombinant plasmid, termed pFHBsAg, was transformed into jM109 competent bacterial cells. The presence of the HBsAg gene was confirmed by PCR screening assay. Moreover,
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