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Digital polymerase chain reaction (PCR) is a quantitative PCR technique with potential to be more accurate and precise than real-time quantitative PCR without the need for a standard curve. The digital PCR sample/reaction mix is randomly distributed into a large number of partitions, such that some partitions contain no copies of the nucleic acid template and others contain at least one copy. Following thermal cycling, partitions containing amplified product can be distinguished by thedoi:10.1071/ma13059 fatcat:4x5hxgndxnbjhndfwogpfje6be