Use of digital micromirror devices as dynamic pinhole arrays for adaptive confocal fluorescence microscopy

Paolo Pozzi, Dean Wilding, Oleg Soloviev, Gleb Vdovin, Michel Verhaegen, Michael R. Douglass, Benjamin L. Lee
2018 Emerging Digital Micromirror Device Based Systems and Applications X  
In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and scanning. A binary DMD is positioned in an image plane of the detection optical path, acting as a dynamic array of reflective confocal pinholes, images by
more » ... a high performance cmos camera. A second camera detects images of the light rejected by the pinholes for sensorless aberration correction.
doi:10.1117/12.2288436 fatcat:5inalig4y5hkvfq35tm5dsjdru