Dietzia psychralcaliphila sp. nov., a novel, facultatively psychrophilic alkaliphile that grows on hydrocarbons

Isao Yumoto, Kiyoshi Kojima, Hidetoshi Matsuyama, Keita Kusumoto, Hideaki Iwata, Akio Nakamura, Yoshinobu Nodasaka
2002 International Journal of Systematic and Evolutionary Microbiology  
A novel, facultatively psychrophilic alkaliphile that grows on a chemically defined medium containing n-alkanes as the sole carbon source was isolated from a drain of a fish product-processing plant. The isolate was an aerobic, non-motile, Gram-positive bacterium. The bacterium was catalase-positive and oxidase-negative. The cell wall contained meso-diaminopimelic acid, arabinose and galactose ; the glycan moiety of the cell wall contained acetyl residues. The GMC content of the DNA was 696 mol
more » ... the DNA was 696 mol %. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate was closely related to members of the genus Dietzia (961-968 % similarity). Comparisons of phenotypic and chemotaxonomic characteristics between the isolate and the two known Dietzia species showed that they were very similar. However, the isolate differed from the two known Dietzia species in growth temperature range and certain physiological characteristics. DNA-DNA hybridization revealed that the isolate had 384 and 497 % relatedness, respectively, to Dietzia maris and Dietzia natronolimnaea. On the basis of the physiological and biochemical characteristics, the phylogenetic position as determined by 16S rRNA gene analysis and DNA-DNA relatedness, it is concluded that the isolate should be designated as a novel species, for which the name Dietzia psychralcaliphila sp. nov. is proposed. The type strain is ILA-1 T (l JCM
doi:10.1099/00207713-52-1-85 pmid:11837320 fatcat:s7qcx6ftange5j2oo3ewym5viu