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Culture of NCI-H441 Cells in Advanced RMPI
This protocol describes the thawing, culturing, and cryopreservation of the human alveolar-like epithelial cell line NCI-H441. NCI-H441 cells should be used in place of the adenocarcinoma cell line, A549, when studying the human alveolar epithelium as, in contrast to A549 cells, H441 cells reflect the in vivo alveolar epithelium barrier and transporter protein function (Ren et al., 2016; Hermanns et al., 2004). Disclaimer: The contents of this article have been reviewed by the US Environmentaldoi:10.21203/rs.3.pex-2011/v1 fatcat:26iajo5p4zhvnpuosuqry6jlua