Nature of the Skin-reactive Principle in Culture Filtrates Prepared from Paracoccidioides brasiliensis

Angela Restrepo-Moreno, John D. Schneidau
1967 Journal of Bacteriology  
Mycelial and yeast-phase culture filtrates prepared from three strains of Paracoccidioides brasiliensis exhibited equal reactivity in sensitized guinea pigs. Ethyl alcohol-precipitated fractions obtained from the culture filtrates also showed no difference in reactivity between mycelial and yeast phase when tested in sensitized guinea pigs. Chemical analyses of the ethyl alcohol-precipitated fractions revealed the presence of seven aliphatic amino acids in both the mycelial-and yeast-phase
more » ... nd yeast-phase products. Glucose, galactose, arabinose, and glucosamine were also detected, but the relative proportions of these sugars were different for the mycelial phase as compared with the yeast phase. Both the mycelial-and yeast-phase ethyl alcohol precipitated 1741 on May 9, 2020 by guest
doi:10.1128/jb.93.6.1741-1748.1967 fatcat:omn746nlqfbtre3kovgu6m5fjy