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Major progress has been achieved in recent years in three-dimensional microscopy techniques. This applies to the life sciences in general, but specifically the neuroscientific field has been a main driver for developments regarding volume imaging. In particular, scanning electron microscopy offers new insights into the organization of cells and tissues by volume imaging methods, such as serial section array tomography, serial block-face imaging or focused ion beam tomography. However, most ofdoi:10.3389/fnana.2018.00112 pmid:30618653 pmcid:PMC6297274 fatcat:ah75wn2edndqvd7nsmxavn54pa