Non-destructive phenotyping of root system architecture can facilitate breeding for root traits that optimize resource acquisition. This protocol describes the construction of a low-cost, highresolution root phenotyping platform, requiring no sophisticated equipment and adaptable to most laboratory and glasshouse environments. The phenotyping platform consists of germination paper abutting scanners and open-source image acquisition software which allows live imaging of root systems. The

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... ing platform is scalable, modular and inexpensive. Materials and Reagents Seeds of the plants to be investigated Note: We used seeds of Brassica rapa L. Nutrient solution (see Recipes) Note: Composition will depend on experimental requirements. Equipment 1. Large (30 x 42 cm) and small (12 x 12 cm) steel blue Anchor seed germination paper Anchor Paper Company, catalog number: SGB1924B) for phenotyping platform and seed germination, respectively 2. Duct tape (office DEPOT) 3. Ferrite magnetic strip with adhesive back (0.75 mm thick) (RS Components, catalog number: 297-9116) 4. Square bioassay or petri dishes (120 mm x 120 mm x 17 mm) (Sigma-Aldrich, catalog number: Z617679) Note: This could be improvised with empty compact disc (CD) cases or any kind of transparent box that meets the specified dimensions.