MICROSCOPY, ANTI-DIARRHOEAL AND REDUCING POWER OF PLANT EXTRACTS OF BOUGAINVILLEA GLABRA "SNOW WHITE"
The aim of this paper was to evaluate microscopy, anti-diarrhoeal and reducing anti-oxidant power of Bougainvillea glabra "Snow White". An animal study (antidiarrhoeal activity) was carried on experimental albino rats. Extract of leaf was extracted by soxhlet apparatus by using hydro alcoholic solvent (50:50). hydroalcoholic extract in 200 mg kg-1 and 400 mg kg-1 doses were administered in two group and loperamide was administered in dose 3mg kg-1 in separate group. After 5 h fecal matter was
... llected and test groups were compared with those in the control animals and analyzed statistically. In the antioxidant activity reducing power of plant extracts were evaluated. Different concentration of plant extract (100-1000 micro litre) were dissolved in 1 ml of water were mixed with phosphate buffer (2.5 ml, 0.2 M, pH 6.6) and potassium ferricyanide [K3Fe (CN) 6 ] (2.5 ml, 1%). The mixture was incubated at 50oC for 20 min. Then, 2.5 ml of trichloroacetic acid (10%) was added to mixture, which was then centrifuged for 10 min at 3000 rpm. The upper layer of solution (2.5 ml) was mixed with distilled water (2.5 ml) and FeCl3 (0.5 ml, 0.1%). The absorbance was measured at 700 nm against a blank using UV-Vis spectrophotometer. The extracts were shown anti diarrhoeal activity and reducing antioxidant power. These activities were statistically significant (P< 0.05) when compared with control. These results suggest that Bougainvillea glabra "Snow White" is able to reduce free radical and effective in bowl imbalance.