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Construction and identification of pIRES2-LIF-NT-3 bicistronic eukaryotic expression vector
2014
Genetics and Molecular Research
We used a simple and efficient method to construct a bicistronic eukaryotic expression vector pIRES 2 -LIF-NT-3. The leukemia inhibitory factor (LIF) and neurotrophin-3 (NT-3) genes were obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The LIF cDNA fragment was inserted into the multiple cloning sites of a vector containing internal ribosome entry site and enhanced green fluorescent protein (EGFP) (pIRES 2 -EGFP) to generate the bicistronic
doi:10.4238/2014.june.18.12
pmid:25036519
fatcat:7mcaoszztjfypb26q7jstkqn3e