Brain pericytes are a major source of lipocalin-type prostaglandin D2 synthase in the cerebral cortex after ischemic stroke

Nahoka MIYATANI, Yoshihiro MOMOTA, Mitsuyo MAEDA, Takayuki NAKAGOMI, Tomohiro MATSUYAMA
Journal of Osaka Dental University  
Lipocalintype prostaglandin D2 Synthase (LPGDS), a major cerebrospinal fluid (CSF) protein known as βtrace, reportedly scavenges biliverdin in the CSF of patients with subarachnoid hemorrhage and functions as a major amyloid βchaperone, suggesting a clearance role in the central nervous system. However, the source of LPGDS in the cere bral cortex following stroke has not been elucidated. We examined changes in the cellu lar localization of LPGDS in poststroke cerebral cortices of mice.
more » ... orescence staining were performed to determine the localization of LPGDS. Immunoelectron micros copy for LPGDS was also performed. In normal cerebral cortices, immunoreactive LPGDS structures were rarely observed. However, higher LPGDS expression levels were ob served on PDGFRβ+ pericytes and nestin+ stem cells. Immunoelectron microscopy confirmed that pericytes in association with the endothelial cells expressed LPGDS. It is strongly expressed in brain pericytes following ischemic insult, suggesting that the ischemic pericytes are the major site of LPGDS expression following stroke. Knowledge of these expression profiles may be useful for future PCbased stem cell therapies. (J Osaka Dent Univ 2020 ; 54 : 9199)
doi:10.18905/jodu.54.1_91 fatcat:qqxk3zjmvvhbzkx5xv4enzeouu