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High-throughput fluorescence-based isolation of live C. elegans larvae
2012
Nature Protocols
For the nematode Caenorhabditis elegans, automated selection of animals of specific genotypes from a mixed pool has become essential for genetic interaction or chemical screens. to date, such selection has been accomplished using specialized instruments. However, access to such dedicated equipment is not common. Here we describe live animal fluorescence-activated cell sorting (laFacs), a protocol for automatic selection of live first larval stage (l1) animals using a standard Facs system. We
doi:10.1038/nprot.2012.084
pmid:22814389
pmcid:PMC5274720
fatcat:yx6nbz3o3zcbjmjn7qpt3boage