Immunohistochemical Detection and Correlation Between MHC Antigen and Cell-mediated Immune System in Recurrent Glioma by APAAP Method

Koichi MIYAGI, Marylou INGRAM, Geza B. TECHY, Dean B. Skip JACQUES, Donald B. FRESHWATER, Hunter SHELDON
1990 Neurologia medico-chirurgica  
As part of an on-going clinical trial of immunotherapy for recurrent malignant gliomas, using alkaline phosphatase-anti-alkaline phosphatase method with monoclonal antibodies, we investigated the cor relation between expression of the major histocompatibility complex (MHC) and the subpopulation of tumor-infiltrating lymphocytes (TILs) in 38 glioma specimens (20 grade IV, 11 grade III, and 7 grade II) from 33 patients. Thirty specimens (78.9%) were positive to class I MHC antigen and 20 (52.6%)
more » ... gen and 20 (52.6%) were positive to class II MHC antigen. The correlations between class I MHC antigen expression and the number of infiltrating T8 (p < 0.01), and also between class II MHC antigen expression and the number of infiltrating T4 (p < 0.05) were significant. We conclude that TILs are the result of immuno reaction (host-defense mechanism). 31.6% of specimens had perivascular infiltration of T cells. The main infiltrating lymphocyte subset in moderate to marked perivascular cuffing was T4. Our results may indicate that lack of MHC antigen on the glioma cell surface has a share in the poor immuno genicity in glioma-bearing patients. In addition, considering the effector⁄target ratio, the number of infiltrating lymphocytes against glioma cells was too small, so the immunological intervention seems to be essential in glioma therapy. Previous radiation therapy and chemotherapy, including steroid therapy, did not influence lymphocyte and macrophage infiltration.
doi:10.2176/nmc.30.649 pmid:1708454 fatcat:t4zdbmztrza3rkpn2krvfwohwm