Molecular basis of isozyme formation of beta-galactosidases in Bacillus stearothermophilus: isolation of two beta-galactosidase genes, bgaA and bgaB

H Hirata, S Negoro, H Okada
1984 Journal of Bacteriology  
Bacillus stearothermophilus IAM11001 produced three ji-galactosidases, ,-galactosidase I, II, and III (0-gal I, II, and III), which are detectable by polyacrylamide (nondenatured) gel electrophoresis. By connecting restriction fragments of the chromosomal lNA to plasmid vectors, followed by transformation of Escherichia coli, two 0-galactosidase genes (bgaA and bgaB) located close to each other on the chromosome were isolated.
doi:10.1128/jb.160.1.9-14.1984 fatcat:zoyfbc5t45a73a654egsgqhtcy