Differentiation of Melipona quadrifasciata L. (Hymenoptera, Apidae, Meliponini) subspecies using cytochrome b PCR-RFLP patterns

Rogério O. Souza, Geraldo Moretto, Maria C. Arias, Marco A. Del Lama
2008 Genetics and Molecular Biology  
Melipona quadrifasciata quadrifasciata and M. quadrifasciata anthidioides are subspecies of M. quadrifasciata, a stingless bee species common in coastal Brazil. These subspecies are discriminated by the yellow stripe pattern of the abdominal tergites. We found Vsp I restriction patterns in the cytochrome b region closely associated to each subspecies in 155 M. quadrifasciata colonies of different geographical origin. This mitochondrial DNA molecular marker facilitates diagnosis of M.
more » ... ata subspecies matrilines and can be used to establish their natural distribution and identify hybrid colonies.
doi:10.1590/s1415-47572008000300009 fatcat:gfhbyz2p2bg5th6f5h3qp7sdhi