G e ne ra l : G e ne ra l : This is an optimized DNA isolation protocol adapted to the properties of bacteria isolated from biogas substrate and digestate, using NucleoBond® AXG Columns and NucleoBond® Buffer Set. The protocol reliably retrieves DNA of sufficient quality, length and yield. The performance has been validated on seven bacterial isolates from one biogas reactor and one biogas plant. Buffers and proteinase K can be found in NucleoBond Buffer Set III. O bse rve d Pe rforma nce on se

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... ve n i sol a te s runs usi ng thi s protocol : O bse rve d Pe rforma nce on se ve n i sol a te s runs usi ng thi s protocol : N50 Ranges between 22,000 and 46,000 bp. General throughput is between 16-22 Gigabases of a single flowcell (flowcell 106 Rev D, using the LSK-109 Kit).