A high yield purification procedure for alpha and beta chains of HLA-DR antigens

L E Walker, R A Reisfeld
1982 Journal of Biological Chemistry  
A procedure is described for purifying to homogeneity the alpha and beta chains of HLA-DR antigen from the homozygous human lymphoid cell line LG-2. The procedure involves a combination of lectin affinity as well as classical and high pressure liquid chromatography. From 100 g of packed cells, 8 mg (235 nmol) of alpha chain and 7 mg (205 nmol) of beta chain were isolated.
pmid:6979540 fatcat:qpspnkhrybdjhnohsrja4422t4