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Concentrations of the intracellular Ca2+-mediator calmodulin (CaM), were measured by radioimmunoassay during heparin-induced capacitation of bull spermatozoa. Heparin reduced sperm CaM concentrations in a dose-dependent manner corresponding with an increase in in-vitro fertilization rates. Such reductions were observed after heparin treatment for 4\p=n-\6 h, which is in agreement with the length of the capacitation period in bulls and was concomitant with an increase in CaM concentration in thedoi:10.1530/jrf.0.0940023 pmid:1552484 fatcat:2qsyv4u72bbrzkowirgp64hwby