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PCR normalization and size selection with magnetic beads v2
[post]
2022
Preserved Fulltext
This protocol describes how to clean up and normalize PCR products or DNA extracts and perform a size selection with carboxylated-magnetic beads and a PEG-NaCl buffer. It works by diluting the beads so that the binding capacity is lower than the PCR yield which leads to a normalization of all PCR products to the binding capacity.
doi:10.17504/protocols.io.q26g7y859gwz/v2
fatcat:fsb3i6vuufctvkcyd3uu2fqzwu