Combined Analysis of mRNA and miRNA Reveals the Banana Potassium Absorption Regulatory Network and Validation of miRNA160a [post]

Wenliang Chen, Tao Dong, Yinglong Chen, Ping Lin, Chuqiao Wang, Kelin Chen, Yi Tang, Mingyuan Wang, Jianfu Liu, Hailing Yu
2022 unpublished
Potassium (K) has an important effect on the growth and development of plants. Banana contains high K content than many other fruits, and its plant requires more K nutrient in soil. However, the soil in the banana-producing areas in China is generally deficient in K. Therefore, understanding the mechanism of banana K absorption may assis in providing effective strategy to solve this problem. This study used two banana varieties with contrasting K tolerance, 'Guijiao No. 1' (low-K tolerant), and
more » ... 'Brazilian banana' (low-K sensitive)to investigate K absorption mechanisms in response to low K stress through miRNA and mRNA sequencing analysis. Under low K condition, 'Guijiao No.1' showed higher plant height, dry weight, tissue K content and ATPase activity. Transcription factor analysis results showed that it was mainly concentrated in MYB, AP2-EREBP, bHLH, etc. The sequencing results showed that 'Guijiao No. 1' had 776 differentially expressed genes (DEGs) and 27 differentially expressed miRNAs (DEMs), and 'Brazilian banana' had 71 DEGs and 14 DEMs. RT-qPCR results showed that all miRNAs and mRNAs showed similar expression patterns with RNA-Seq and transcriptome. miRNA regulatory network was constructed by integrated analysis of miRNA-mRNA data. miR160a was screened out as a key miRNA, and preliminary functional validation was performed. Arabidopsis overexpressed miRNA160a reduced tolerance to low K, and inhibited phenotypical traits such as root length, and reduced K accumulation. The overexpressed miR160a had a targeting relationship with ARF10 and ARF16 in Arabidopsis. These results indicate that miRNA160a may regulate K absorption in bananas through the auxin pathway. This study provides a theoretical basis for further research on the molecular mechanism underlying the response of banana plants to low-K stress and for molecular breeding.
doi:10.21203/ fatcat:t3zsmws2vrbxzp5aiz5jbqr5zu