Identification and Laboratory Fungicides Screening of the Pathogenic Fungus of Stem Spot of Pitaya (Hylocereus spp.) Stems

Hui Luo, Kun Guo, Xueying Shang, Lei Peng, Zhijun Peng, Jilin Jin, Bin Wang, Xingwu Zhang
2022 Journal of Chemistry  
In this study, the pathogenic fungus of stem spot disease of pitaya (Hylocereus spp.) stems was identified by isolation and purification, pathogenicity test, morphological characteristics, and analysis of rDNA-ITS sequences. The results turned out that the rDNA-ITS sequences of the H1 strain showed 100% of identity with Botryosphaeria dothidea, indicating that the pathogenic fungus of stem spot disease of pitaya stems was Botryosphaeria dothidea. Meanwhile, the H1 strain was then used as a
more » ... ence strain to screen some commercial fungicides. The bioassay test results indicated that prochloraz had an obvious inhibitory effect on Botryosphaeria dothidea with the EC50 value of 0.0798 μg/mL. Our study could provide a theoretical basis for the effective control method of stem spot disease of pitaya.
doi:10.1155/2022/3546266 doaj:258df36c2afc4c6e886ba91a0de19157 fatcat:sckteo7pn5ff3aldhbinzkswgy