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We present a multi-color STED fluorescence microscope providing far-field optical resolution down to 20 nm for biomedical research. The optical design comprises fiber lasers, beam scanners, and a set of active and passive polarizing elements that cooperatively yield an optically robust system for routinely imaging samples at subdiffraction length scales.doi:10.2528/pier14042708 fatcat:tqmsyoqu7zhoddvxngdicdnefa