New anti-candida active nitrogen-containing bisphosphonates as inhibitors of farnesyl pyrophosphate synthase Candida albicans

L. O. Metelytsia, D. M. Hodyna, O. L. Kobzar, V. V. Kovalishyn, I. V. Semenyuta
2019 Ukrainian Biochemical Journal  
In our previous work, a number of new nitrogen-containing bisphosphonates (N-BPs) with high predicted and experimental antifungal activity were presented as potential Candida albicans farnesyl pyrophosphate synthase (FPPS) inhibitors. To confirm this hypothesis, a homologous C. albicans FPPS model with high-quality scores has been developed and used in present work to study the molecular mechanism of nitrogen-containing bisphosphonates action as anti-Candida agents. the known FPPS inhibitors
more » ... FPPS inhibitors ammonium 2-(Pyridin-2-ylamino)ethylidene-1,1-bisphosphonate, risedronate and alendronate were used in molecular docking analysis. the molecular docking analysis of the new N-BPs demonstrated a number of common features of all ligand's interaction in the active center of FPPS C. albicans. It is established that the ligands phosphonate groups are the key elements in the formation of the stable ligand-protein complexes with binding energy in a range (ΔG) from -6.6 to -7.1 kcal/mol due to a significant number of electrostatic, hydrogen and metal-acceptor bonds. It is confirmed that the new studied N-BPs 1 and 3 with high anti-Candida activity are FPPS inhibitors. K e y w o r d s: nitrogen-containing bisphosphonates, farnesyl pyrophosphate synthase, Candida albicans, homology modeling, molecular docking.
doi:10.15407/ubj91.03.078 fatcat:fpagu65kwfd3tcx2rqps4rmtnu