Pfaffi a glomerata (Spreng.) Pedersen, Amaranthaceae, is widely distributed in Brazil. Roots are considered as the world's greatest supplier and β-ecdysone is the most important compound extracted from roots of Pfaffi a glomerata. So, the aim this study was analyze the presence of β-ecdysone in the infl orescences and stems and compared with the content from roots of Pfaffi a glomerata and determine the best extractive method of β-ecdysone this plant. The crude extracts were obtained by Soxhlet

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... method, refl ux, maceration, percolation and turbolyse. Compound extracts were quantifi ed by High Performance Liquid Chromatography (HPLC). The analysis were carried out a Phenomenex Column C18, 5 µm, 250x4,6mm, maintened at 30 o C, gradient system using as mobile phase a mixture of methanol and water, fl ow rate 1,0 mL and detection at 245 nm. Results showed Soxhlet method with ethanol:water (90:10 v/v) presented the higher concentration of β-ecdysone in P. glomerata and infl orescences showed higher amount of this active substance (3,06%), compared with stems (2,37%) and roots (1,63%), showing that the infl orescences and plant stems may also be used as a rich source of β-ecdysone. Keywords: Pfaffia glomerata β-ecdysone extraction HPLC Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC Lara Z. Serra et al. Rev. Bras. Farmacogn. Braz. J. Pharmacogn. 22(6): Nov./Dec. 2012