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Structural basis of transcription inhibition by the DNA mimic protein Ocr of bacteriophage T7
Bacteriophage T7 infects Escherichia coli and evades the host defence system. The Ocr protein of T7 was shown to exist as a dimer mimicking DNA and to bind to host restriction enzymes, thus preventing the degradation of the viral genome by the host. Here we report that Ocr can also inhibit host transcription by directly binding to bacterial RNA polymerase (RNAP) and competing with the recruitment of RNAP by sigma factors. Using cryo electron microscopy, we determined the structures of Ocr bounddoi:10.1101/822460 fatcat:albgo3wcorhrbicoosmm4rxvgq