Bombesin-like immunoreactivity in mammalian tissues

CHIZUKO YANAIHARA, ATSURO INOUE, TOHRU MOCHIZUKI, JUNKO OZAKI, HARUKO SATO, NOBORU YANAIHARA
1980 Biomedical research  
Antiserum directed to the amphibian bombesin was raised in a guinea pig using the conjugate of N"-glycyl-[1-Glutamine]-bombesin with bovine serum albumin. The major immunologic determinant of this antiserum GP-3303 was shown to reside in the 6-7 sequence of bombesin. Bombesin-like immunoreactivities in gastrointestinal tissue extracts of the pig, dog, monkey, rat and guinea pig were found to be extremely low when measured by the present radioimmunoassay system using antiserum GP-3303. The
more » ... e gastrin releasing peptide recently isolated has the same decapeptide sequence as bombesin (5-14) in the C-terminal region except for the amino acid in position 7 of the bombesin molecule. The very low concentrations of bombesin-like immunoreactivity in the mammalian tissue extracts examined in the present study seem to reflect, at least in part, this amino acid substitution in the position corresponding to position 7 of bombesin, since the amino acid is involved in the major immunologic determinant of antiserum GP-3303. The present result also suggests that the bombesin-like immunoreactivity detected in mammalian tissues may be due to a new group of peptides in which the porcine gastrin releasing peptide is included. KEY WORDS bombesin-like immunoreactivity / porcine gastrin releasing peptide / radioimmunoassay
doi:10.2220/biomedres.1.96 fatcat:udn5gl5i6vcalfzdsduebpb6yu