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Quantitative Determination of Total L-Carnitine in Infant Formula, Follow-up Formula, and Raw Materials by Liquid Chromatography with Tandem Mass Spectrometry
2014
Analytical Sciences
We developed a rapid and useful routine screening assay for total L-carnitine content in various infant formulas and materials by liquid chromatography coupled with a tandem mass spectrometric method (LC-MS/MS) and alkaline hydrolysis. For separation of L-carnitine, a multi-mode octadecylsilane (ODS) column was used that contained ODS ligands, anion ligands, and cation ligands to avoid using ion-pairing agents. The stable isotope L-carnitine-d3 (m/z 165 → 103/85) was used in electrospray MS/MS
doi:10.2116/analsci.30.839
pmid:25109647
fatcat:h53u3pcrdjf4dbq5cdi2sxkufm