Quantitative Cerebral Blood Flow Measurements in the Rat Using a Beta-Probe and H215O

Bruno Weber, Nicolas Späth, Matthias Wyss, Damian Wild, Cyrill Burger, Ross Stanley, Alfred Buck
2003 Journal of Cerebral Blood Flow and Metabolism  
Beta-probes are a relatively new tool for tracer kinetic studies in animals. They are highly suited to evaluate new positron emission tomography tracers or measure physiologic parameters at rest and after some kind of stimulation or intervention. In many of these experiments, the knowledge of CBF is highly important. Thus, the purpose of this study was to evaluate the method of CBF measurements using a beta-probe and H 2 15 O. CBF was measured in the barrel cortex of eight rats at baseline and
more » ... fter acetazolamide challenge. Trigeminal nerve stimulation was additionally performed in five animals. In each category, three injections of 250 to 300 MBq H 2 15 O were performed at 10-minute intervals. Data were analyzed using a standard one-tissue compartment model (K 1 ‫ס‬ CBF, k 2 ‫ס‬
doi:10.1097/01.wcb.0000095799.98378.7d pmid:14663341 fatcat:uoyvcmeye5aqbk5qakdhj4ryqu