A natural agent that, promotes osteogenic differentiation of dental pulp mesenchymal stem cells (DP-MSCs) could achieve success in regeneration during healing and may also prevent bone resorption and improve regeneration. We aimed to demonstrate that a Nigella sativa oil could induce differentiation of DP-MSCs. DP-MSCs were isolated from 3. molars and identified with flow cytometer. Osteogenic differentiation was conducted, and calcium granules were showed by typical Alizarin Red dying and

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... um concentration were determined by DICA 500. Identification results showed that the cells are mesenchymal stem cells. Alizarin Red dying signed the calcium granules and DICA 500 showed that calcium concentrations were higher than the Control Group. This preliminary study shows for the first time the inductive potential of N. sativa oil for osteogenic differentiation in DP-MSCs. The mechanism should be investigated in further studies.