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The elemental composition of melanin granules and other components of the hair shaft was determined by multiisotope imaging mass spectrometry, a method with unique advantages for the visualization and quantification of stable isotopes and the elemental composition in study of the fine structure of biologic samples. We mapped and quantified the chemical composition of hair cross-sections using secondary ions generated from naturally occurring 16 O, 12 C 14 N, 32 S, and 34 S with a maximumdoi:10.1046/j.0022-202x.2004.22217.x pmid:15009719 fatcat:kdiv2uwimbfw7p7kxvhshxrfgi