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Cu2+ regulated sulfonamides resistance gene (sul) via reactive oxygen species induced ArcA in a pathogenic Vibrio splendidus
Annals of Microbiology
Purpose To detect the sulfonamide resistance of Vibrio splendidus strain Vs, and characterize the factors that regulate the expression of sulfonamide resistance gene sul Vs . Methods Minimal inhibitory concentration was measured using growth inhibition method, gene expression was measured using real-time reverse transcriptase PCR, reactive oxygen species (ROS) production under Cu 2+ or Cu 2+ /NAC conditions was measured using an excitation wavelength of 485 nm, and an emission wavelength of 525doi:10.1007/s13213-019-01475-6 fatcat:cwj6kyozojcupmj6q7mmxc7bku