Cu2+ regulated sulfonamides resistance gene (sul) via reactive oxygen species induced ArcA in a pathogenic Vibrio splendidus

Huijie Liu, Tongxiang Song, Tengteng Lv, Xuelin Zhao, Yina Shao, Chenghua Li, Weiwei Zhang
2019 Annals of Microbiology  
Purpose To detect the sulfonamide resistance of Vibrio splendidus strain Vs, and characterize the factors that regulate the expression of sulfonamide resistance gene sul Vs . Methods Minimal inhibitory concentration was measured using growth inhibition method, gene expression was measured using real-time reverse transcriptase PCR, reactive oxygen species (ROS) production under Cu 2+ or Cu 2+ /NAC conditions was measured using an excitation wavelength of 485 nm, and an emission wavelength of 525
more » ... nm, and the binding of sul Vs promoter and the transcriptional factor ArcA was measured using electrophoretic mobility shift assay (EMSA). Result sul Vs gene was cloned and its expression was upregulated to 6.98-and 3.57-fold in the presence of sulfadiazine and sulfamethoxazole, respectively. Moreover, Cu 2+ could also upregulate the expression of sul Vs to 14.27-fold, the production of ROS to 4.37-fold, and the expressions of antioxidant-related genes and a transcriptional regulator arcA gene. After addition of N-Acetyl-L-cysteine, a ROS inhibitor, the production of ROS decreased to 53.1%, and the expression of arcA gene was also downregulated to 26%. EMSA showed that the purified recombinant ArcA could directly bind to the promoter region of sul Vs with specificity. Conclusion V. splendidus strain Vs showed sulfonamide resistance due to sul Vs , and Cu 2+ could increase the level of ROS followed by ArcA activation as a transcriptional factor to increase the expression of sul Vs .
doi:10.1007/s13213-019-01475-6 fatcat:cwj6kyozojcupmj6q7mmxc7bku