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Kinetic analysis of Drosophila Vnd protein containing homeodomain with its target sequence
2010
BMB Reports
Homeodomain (HD) is a highly conserved DNA-binding domain composed of helix-turn-helix motif. Drosophila Vnd (Ventral nervous system defective) containing HD acts as a regulator to either enhance or suppress gene expression upon binding to its target sequence. In this study, kinetic analysis of Vnd binding to DNA was performed. The result demonstrates that DNA-binding affinity of the recombinant protein containing HD and NK2-specific domain (NK2-SD) was higher than that of the full-length Vnd.
doi:10.3858/bmbrep.2010.43.6.407
fatcat:2yryeayeezbidcul5hvi6ua55e