Large Rhizosphere Bacterial Diversity Exits Among Wild Progenitor Species of Modern Sugarcane (Saccharum Spp. Inter-Specific Hybrids)
BackgroundRhizosphere is rich in highly diverse and complex microbial communities. Plant growth promoting rhizpbacteria and diazotrops are played crucial role in plant growth and development. In this study, rhizosphere soils were collected from five wild Saccharum species- S. officinarum L. cv Badila (BRS), S. barberi Jesw. cv Pansahi (PRS), S. robustum (RRS), S. spontaneum (SRS), and S. sinense Roxb. cv Uba (URS) for studied of rhizosphere and diazotroph bacterial diversity using 16S rRNA and
... ifH gene amplification and sequencing.ResultsWe detected a total of 6202 operational taxonomic units (OTUs) specific to the bacterial communities from all species combined. Out of the 107 bacterial communities detected among all samples, we found a core microbiome of 31 rhizobacterial families spread across all the species analyzed. A total of 1099 OTUs were identified for diazotrophs with a core microbiome of 9 families distributed among all the sugarcane species. The core microbiomes were distributed across twenty genera-Bradyrhizobium, Dechloromonas, Desulfovibrio, Stenotrophomonas, Xanthobacter, Anaeromyxobacter, Azospirillum, Pseudoacidovorax, Methylobacterium, Azoarcus, Paenibacillus, Ideonella, Beijerinckia, Paraburkholderia, Burkholderia, Ruficoccus, Geobacter, Sinorhizobium, Kosakonia, and Azotobacter. ConclusionThe results presented here advance our understanding of rhizosphere associated bacterial diversity among genetically closely related wild species and provide a knowledge base for studying the evolution of rhizobacteria-host plant association during crop domestication.