Application of the Direct Colony TLC Method for Identification of Phytopathogenic Bacteria

Nobuaki Matsuyama, Ismail Hossain Mian, Abdul Mannan Akanda, Naruto Furuya
1993 Journal of the Faculty of Agriculture Kyushu University  
Direct colony thin-layer chromatography was applied for the identification of the phytopathogenic bacteria. This method involves two steps such as the extraction of lipids from bacterial cells and their development. Chloroform-methanol (2:1, v/v) and chloroform-methanol-5M ammonia (60:25:4, v/v) were used as the solvents for the first and second developments, respectively. Omission of the first step and the direct development with the second solvent gave worse results with tailing. Inter-genus
more » ... ifferences, at least, were observed among the chromatograms. Striking difference of chromatogram was observed between Clavibacter spp. (Gram positive) and others (Gram negative). The chromatograms of Erwinia spp. were characteristic and quite different from those of Xanthomonas spp. and Agrobacterium tumefaciens. Although some difficulties like the cases of Pseudomonas spp. are still remaining, the practical usefulness of this method for a rapid identification of phytopathogenic bacteria was certainly verified.
doi:10.5109/24020 fatcat:ukmfaokcjnclzks2ztipr2itzm