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Application of the Direct Colony TLC Method for Identification of Phytopathogenic Bacteria
1993
Journal of the Faculty of Agriculture Kyushu University
Direct colony thin-layer chromatography was applied for the identification of the phytopathogenic bacteria. This method involves two steps such as the extraction of lipids from bacterial cells and their development. Chloroform-methanol (2:1, v/v) and chloroform-methanol-5M ammonia (60:25:4, v/v) were used as the solvents for the first and second developments, respectively. Omission of the first step and the direct development with the second solvent gave worse results with tailing. Inter-genus
doi:10.5109/24020
fatcat:ukmfaokcjnclzks2ztipr2itzm