This study was performed to investigate the effects of isoprothiolane on cell growth and the production of interleukin (IL)-1 and IL-6 by bovine mammary epithelial cells in vitro. Isoprothiolane increased proliferation of mammary epithelial cells in a dosedependent manner at the concentration of 0.05 to 5 µM when cultured either with or without serum-supplemented medium. In contrast, isoprothiolane (0.0005-5 µM) significantly inhibited the production of IL-1 and IL-6 by mammary epithelial

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... Moreover, the cytokines, IL-1α, IL-1β, IL-6, and tumor necrosis factor (TNF)-α tended to inhibit the proliferation of mammary epithelial cells in a dosedependent manner. These results indicated that isoprothiolane regulated mammary epithelial cell growth in vitro possibly by modulating the production of cytokines.-KEY WORDS: cytokine, isoprothiolane, mammary gland.