Interleukin-3Rα+ Myeloid Dendritic Cells and Mast Cells Develop Simultaneously from Different Bone Marrow Precursors in Cultures with Interleukin-3

Thomas Baumeister, Susanne Rößner, Gabi Pech, Gerold Schuler, Manfred B. Lutz, Marella F.T.R. de Bruijn, Pieter J.M. Leenen
2003 Journal of Investigative Dermatology  
The distinct developmental routes of dendritic cells and mast cells from murine bone marrow cultures with interleukin-3 are unclear. We found that short-term bone marrow cultures with interleukin-3 after 8^10 d consist of about 10%^30% dendritic cells and 70%^90% mast cell precursors, and only after 4^6 wk do homogeneous populations of mast cells emerge. Phenotypical and functional analysis of interleukin-3/dendritic cells revealed a high similarity with myeloid dendritic cells generated with
more » ... anulocyte-macrophage colony stimulating factor in the expression of myeloid dendritic cell markers (CD11c þ B220^CD8a^CD11b þ ), major histocompatibility complex II and costimulatory molecules, endocytosis, maturation potential, interleukin-12 production, and T cell priming. Interleukin-3/dendritic cells expressed higher levels of interleukin-3 receptor, however. To dissect the interleukin-3/dendritic cell and mast cell development, we sorted fresh bone marrow cells into six subsets by the antibodies ER-MP12
doi:10.1046/j.1523-1747.2003.12380.x pmid:12880419 fatcat:2o5e7pukz5gnvczmwwlgarhxj4