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Understanding microchannel culture: parameters involved in soluble factor signaling
Lab on a Chip
While the importance of autocrine-paracrine signaling in vivo is clear, the ability to study the effects of secreted endogenous factors in vitro is hampered by canonical culture platforms. In multi-well plates, the large air-liquid interface gives rise to convective flows that continually mix the fluid disrupting the local diffusion-based accumulation. Simple microchannels provide a more controlled microenvironment that can be used to study secreted factor effects. Here, we utilize microchanneldoi:10.1039/b618793e pmid:17538714 fatcat:opmumyugj5glvld3pqcugjg5ue