One simple and sensitive procedure (simple spectrophotometric method) for the assay of drug clarithromycin in pure form and formulations. This method involves the formation of ion-association complex between CAM and the TPOOO, ARS and WFB. In order to establish the optimum conditions necessary for rapid and quantitative formation of coloured product with maximum stability and sensitivity, the author performed experiments by measuring the absorbance at λmax 480nm,420nm and580nm of respective

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... es of solutions, varying one and fixing the other parameters in each case such as type, volume and concentration of acid, organic solvent used for extraction, ratio of organic phase to aqueous phase during extraction, shaking time and temperature. The variable parameters were optimized. The results were statistically validated.