Genomic analysis of polyaromatic hydrocarbon degrading Paenibacillus species

Ahmed Al-Shiti
Polyaromatic hydrocarbons (PAHs) are a group of complex organic compounds that contain at least two aromatic rings. Some of the PAHs, which have high molecular weight, are known carcinogens. PAHs are one of the most prevalent pollutants in the environment and have two main sources: petroleum products and the incomplete combustion of wood. Several microorganisms are known for the ability to degrade PAHs, either aerobically or anaerobically, such as algae, bacteria, and fungi. The initial step of
more » ... The initial step of the aerobic degradation of PAHs is catalyzed by dioxygenase enzymes, which introduces oxygen atoms into the aromatic rings. The first aim of this study was to isolate and identify bacterial species that may have unique enzymes for PAH degradation. Two strains, Paenibacillus validus RD5 and RD6, were isolated from sediment samples obtained from the Logan River, Utah, USA, by aerobic enrichment using biphenyl as the sole carbon source. The second aim was to sequence the RD5 and RD6 genomes and examine transcription of several predicted dioxygenase genes in P. validus RD5 and P. naphthalenovorans PRN1 during growth on naphthalene, biphenyl, dibenzofuran, cinnamate, and benzoate using the reverse transcription polymerase chain reaction (RT-PCR) technique. Based on the RT-PCR results and comparison of the dioxygenase sequences to other enzyme sequences in GenBank hypotheses were formulated about the role of three of the enzymes in biphenyl, naphthalene, and dibenzofuran degradation in the two Paenibacillus strains
doi:10.7282/t3hd7zfz fatcat:card77dcdncxdk5qjgo6wknsou