The coupling of size-exclusion HPLC with ICP-MS in bioinorganic analysis

A. Makarov, J. Szpunar
1998 Analusis  
T he detection of a metal compound in a sample is the prerequisite of any further action concerning its identification, characterisation and the role in biochemistry. The actual problem can be reduced to proving that the analytical signal detected by the spectrometer is due to the presence of a metal compound and not to that of a simple ion. This can be done by preceding the detection by a separation technique that would differentiate between the free metal or metalloid ion and the same element
more » ... nd the same element bound with a larger, usually macromolecular structure. M 44 ANALUSIS MAGAZINE, 1998, 26, N° 6 Figure 1. SEC-HPLC-ICP-MS chromatogram of Al in cytosol of neuroblastoma cells. Superdex-75 HR (30 cm × 10 mm × 13 µm), eluent: 30 mM Tris-HCl buffer, pH 7.2. M 46 ANALUSIS MAGAZINE, 1998, 26, N° 6
doi:10.1051/analusis:199826060044 fatcat:lz5xqo76tjg63iy3m5ndpopjsu