A study is made of the effects of Doppler broadening on pure gain without inversion, which means that neither one-photon nor two-photon inversions are allowed, and non-pure gain without inversion, which means that one-photon inversion does not occur but two-photon inversion is present, in a closed Λ-type three-level system with incoherent pumping. It is shown that when the driving field is resonant but the probe field is not, in a certain range of Doppler width, for the case of the lower degree

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... of frequency up-conversion, generally, pure gain without inversion increases monotonically and non-pure gain without inversion does not monotonically increase or decrease with increasing Doppler width; for the case of the higher degree of frequency up-conversion, pure gain without inversion decreases monotonically but non-pure gain without inversion cannot be produced. In the case of two-photon resonance, in some range of Doppler width, pure gain without inversion does not monotonically increase or decrease while non-pure gain without inversion decreases monotonically with Doppler width increasing. Finally, an experimental scheme for examining our theoretical result is given.