Computational studies on Gene Ontology for Molecular functions, Cellular component and Biological process of SARS-CoV-2 targeted proteins [post]

Hemanth kumar Manikyam
2020 unpublished
World is facing pandemic COVID-19 caused by SARS-CoV-2.Researchers are working to identify potential cure and treatment for COVID-19. Many Repurposed drugs been proposed to treat the infection with good success rate in some cases. But still there is an urgency to discover potential cure and treatment. In order to discover potential cure we should understand the Gene Ontology of targeted proteins of SARS-CoV-2 for their Molecular functions, cellular component and Biological process. Spike
more » ... ocess. Spike glycoproteins, Main protease (Mpro), Papain like protease (PLpro) are three main proteins that aid viral host cell entry and its replication causing pathogenesis. Using MetaGO an algorithm an algorithm for predicting Gene Ontology (GO) of proteins the Molecular functions, Cellular component and Biological process of SARS-CoV-2 proteins studied. MetaGO analysis had predicted Spike protein gene ontology functions include host cell surface binding, receptor binding, host cell surface receptor binding, structural molecule activity, carbohydrate binding. Main Protease (Mpro) and Papain like protease (PLpro) gene ontology functions predicted hydrolase activity, RNA-directed RNA polymerase activity, ubiquitinyl hydrolase activity, Lys48-specific deubiquitinase activity, mRNA (nucleoside-2'-O-)-methyltransferase activity, mRNA (guanine-N7-)-methyltransferase activity, double-stranded RNA binding, 3'-5'-exoribonuclease activity, helicase activity, single-stranded RNA binding, cysteine-type endopeptidase activity, omega peptidase activity, zinc ion binding, thiol-dependent ubiquitinyl hydrolase activity, ATP binding, endonuclease activity among which PLpro had shown inhibition of ISG15 activity in additional. Inhibition of ISG15 indicates down regulation of interferon production particularly Interferon Type 1. PYCR1 (Pyrroline-5-carboxylate reductase 1) which protects the cells from mitochondrial damage due to oxidative stress shown to be inhibited by Spike protein hydrolase like activity and leading to cell death.PYCR1 can be a diagnostic tool to detect novel coronavirus infection. Viral proteins also use cellular Ubiquitinyl enzymes for its replication and survival in host cell. Thus through gene ontology studies we propose use of Interferon type 1 therapy against COVID-19 caused by SARS-CoV-2.
doi:10.21203/rs.3.rs-26263/v1 fatcat:yntfox5pffay7c33hdlrciivge