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Inclusion complex of acetanilide (AA) with β-cyclodextrin (β-CD) has been investigated by UV and Fluorescence techniques. The red shift in λmax and enhanced absorption in β-CD medium confirms the-NH-CO-CH 3 aromatic side chain and part of benzene ring of AA is inserted in the β-CD cavity. The formation of complex has been confirmed by Benesi-Hildebrand plot obtained from the results of UV and fluorescence studies. The pKa values of prototropic species, stability constant K1:1 and thefatcat:7apw6ukssjfrlne3ohzoegb24u