Ancient human parvovirus B19 in Eurasia reveals its long-term association with humans

Barbara Mühlemann, Ashot Margaryan, Peter De Barros Damgaard, Morten E Allentoft, Lasse Vinner, Anders J Hansen, Andrzej Weber, Vladimir I Bazaliiskii, Martyna Molak, Jette Arneborg, Wieslaw Bogdanowicz, Ceri Falys (+15 others)
Human parvovirus B19 (B19V) is a ubiquitous human pathogen associated with a number of conditions, such as fifth disease in children and arthritis and arthralgias in adults. B19V is thought to evolve exceptionally rapidly among DNA viruses, with substitution rates previously estimated to be closer to those typical of RNA viruses. Based on genetic sequences up to ~70 years of age, the most recent common ancestor (MRCA) of all B19V has been dated to the early 1800s and it has been suggested that
more » ... een suggested that genotype 1, the most common B19V genotype, only started circulating in the 1960s. Here we present 10 genomes (63.9% to 99.7% genome coverage) of B19V from dental and skeletal remains of individuals who lived in Eurasia and Greenland from ~0.5 to ~6.8 thousand years ago (kya). Five ancient B19V sequences fall within or basal to modern genotype 1 and five fall basal to genotype 2, showing a long-term association of B19V with humans. We find a substitution rate of the virus that is an order of magnitude lower than inferred previously. The most recent common ancestor of all B19V is placed ~10.7 kya. Further, we are able to date the recombination event between genotypes 1 and 3 that formed genotype 2 to approximately 5.1 to 6.7 kya. This study presents some of the oldest exogenous viral sequences yet found and emphasizes the importance of ancient viral sequences for our understanding of virus evolution and phylogenetics.
doi:10.17863/cam.26964 fatcat:gilv54bhpbcdhd4beb3xusto3q