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Purification and proteolysis of vesicles containing inside-out and right-side-out oriented reconstituted (Na+, K+)-ATPase
1984
Journal of Biological Chemistry
The (Na+,K+)-ATPase from dog kidney has been reconstituted into egg lecithin vesicles (Goldin, S. M. (1977) J. Biol. Chem. 252, 5630-5642). Using sucrose density gradient centrifugation, we have isolated sealed vesicle populations in which the protein molecules have defined orientations. Sealed vesicles sedimented at higher density than unsealed vesicles after equilibration with CsCl. Vesicles containing inside-out oriented enzyme sedimented at lower density than vesicles containing
pmid:6325427
fatcat:cgeqfyqzj5gelkxe2mfcpgoc2e