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In the current work, iron nanoparticles (Fe-NPs) were prepared from extracts of Ruellia tuberosa L. extracted with ethanol and water, and their antibacterial activities toward pathogenic bacteria (S. aureus and E. coli) were investigated. The morphologies, structures, sizes and distribution of atom on the surface of Fe-NPs were checked by SEM-EDS, UV-Vis and FTIR spectroscopy. The pH conditions (3, 5, 7, and 9) were chosen as experimental factors for the preparation of Fe-NPs. Formation ofdoi:10.31788/rjc.2020.1315511 fatcat:c6azwpswxngr7ps74yn2y3yzha